multiphoton Videos

Confocal and multiphoton microscopy are non-invasive fluorescent imaging techniques that use lasers of different wavelengths to excite the sample and produce the image. Watch the video to find out more and see a confocal microscope in action. For more information see www.wellcomeimageawards.org

Author: WellcomeCollection; Tags: confocal multiphoton microscopy flourescent imaging wellcome image awards collection lasers stuart townsend harwell MRC

spectroscopy/microscopy. Determination of new applications for Focused Ion / Electron Beam systems towards the study of biological systems using near-field and far-field microscopies. Derek Nowak Physics Research Odyssey Near-Field Microscopy Multi-Photon Imaging ANSOM Project FDTD Modeling MPI Clustering LMIS Development Classes Near-Field Microscopy Motivation: To obtain florescence images of biological and material systems at spatial resolutions significantly lower than the ...

Author: delmarphotonics; Tags: femtosecond NSOM Near Field Optical Imaging System

I do a TTF with a level 1 HUmar and PB the Dragon 4 times. Similar hilarity ensues with De Rol Le. Recorded with FRAPS. Video compressed with VirtualDub. Audio mixed with Audacity. Audio Compressed with RazorLame. Song: LIVE.ogg in PSO\data\ogg Of course, the game is Phantasy Star Online run on Schthack server.

Author: RipleySawzen; Tags: PSO Phantasy Star Online Dragon HUmar De Rol Le PB Photon Blast

The UCLA Biophysics Neuroimaging Laboratory is committed to developing new microscopy techniques to further the reach of experimental methods in the area of systems neuroscience. The Lab is collaborating to develop next generation multi-photon microscopy systems, with the aim of imaging millimeter volumes of tissue with non-linear enhanced resolution at millisecond time scales. This may improve methods for analysis of in-vivo cortical circuits and classic microcircuit systems via calcium ...

Author: delmarphotonics; Tags: UCLA Biophysics Neuroimaging Laboratory microscopy neuroscience multi-photon imaging tissue non-linear enhanced resolution in-vivo cortical calcium potentiometric nerve cells fluorescence Knudsen physics ultrafast Ti:Sa oscillator Del Mar Ventures Photonics coherent laser spectrum spectra newport optical table Modelocked KLM Mode-locking bandwidth avalanche photodiode lock-in amplifier zebra fish two-photon three-photon confocal photon zebrafish axotomy time-lapse

Movie showing image processing, cell isolation, and cell tracking of an example movie from a multiphoton microscope.

Author: BuschmanTim; Tags: CellTrackingExampleMovie

Mounted artery of the mouse. 3 channel overlay with autofluorescence of elastin (blue), syto for nuclei of cells in the vascular wall (green/white), Eosin auro-fluorescence (red) Imaging depth: 650 microns Movie acquired with Leica TCS SP5 MP - DM6000 CFS Courtesy of Marc von Zandvoort, Biophysics, University of Maastricht, Netherlands

Author: LEICACLSM; Tags: multiphoton multi-photon multo photon microscopy confocal Leica fluorescence SP5

Specimen: danio rerio Treatment: RNAinj Fishline: WT/TU x WT/Tu Microscope: Leica TCS SP5 Confocal and Multiphoton Microscope with conventional scanner Excitation: t-Pulse Amplitude 1030nm +980 (mai tai) Objective: 20x/0,95NA W Olympus Zoom: 1x Room temperature: 22 degrees C Speed: 200 Hz Channels (n, g): 0,1 averaging Imaging scheme: Voxel: 1.37 x 1.37 x 1.37 μ³ Type: L4 Volume: 512x512x139 px³ (420 stacks) 699.9x699.9x189.06 μ³ Duration: 20:39:59 h 640 (2:57 min 142 between stacks) Stage 0 ...

Author: LEICACLSM; Tags: confocal imaging Leica TCS SP5 danio rerio fluorescence microscopy medizin biology Microsystems

24x speed, with entire sequence constituting four minute tracked acquisition. Amount of motion compensation for this experiment is shown in Figure 5. At t=0, nitrogen replaces oxygen in the ventilator and the onset of systemic hypoxia occurs during the first minute. Upper frames show both channels raw acquisition from tracking microscope, while lower frames show in-plane motion corrected output from retrospective deformable image registration algorithm. ... microscope motion correction ...

Author: myextrafile5; Tags: microscope motion correction multiphoton

Raw image data from rendering of Video 3, acquired as stack spanning 150 micrometers. Showing vasculature dyed with di-8-ANEP-PS (red) and auto-fluorescent NAD(P)H (green). ... microscope muscle multiphoton 3D

Author: myextrafile5; Tags: microscope muscle multiphoton 3D

Data from NAD(P)H channel of the example experiment shown in Video 1. Upper frame shows image sequence after filtering out non-significant components of the principal component analysis (PCA), as described in Detailed Methods. Lower frame shows PCA reconstructed data as a ratio to first-minute-average starting condition, with a grayscale linear lookup table of -50% (black) to +400% (white). ... microscope motion correction multiphoton

Author: myextrafile5; Tags: microscope motion correction multiphoton